IB Bussen: Lab Report: Enzyme Activity


enzyme activity lab report

The Effect of pH on the Rate of Enzyme Catalysis of Catalase. The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. The activity of enzymes is affected by both the concentrations of enzymes present and the concentration of substrate present. As. May 21,  · Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi KC Introduction: An Enzyme is a protein, which is capable of starting a chemical reaction, which involves the formation or breakage of chemical bonds. A substrate is the . This lab shows the affects these factors have on the rate of reaction between catalase, an enzyme found in potatoes, and hydrogen peroxide, the substrate. The specific enzyme that was studied during this lab was catalase. Catalase is a naturally occurring enzyme that is found in many living organisms such as plants and the human body.

The Effect of low pH on Enzyme Activity Lab Report

Chemical reaction in general can be accelerated in a number of ways. Two important ways are by an increase in temperature up to the optimum and by the use of catalysts. A catalyst is a substance that increases the rate of a reaction without being used up itself.

Enzymes are protein molecules that function as catalysts and speed up reactions in living organisms without the need to raise the temperature of the reaction Enzymes are usually specific for particular reactions and work best when conditions such as pH, enzyme activity lab report, temperature and ion concentration are most suitable.

If these conditions are inappropriate for that enzyme, the reactions will proceed at a slower rate or not at all. Hydrogen peroxide H 2 O 2 is a very reactive chemical that is formed as a by-product in cellular reactions.

It is highly toxic and must be removed or it will disrupt chemical reactions in the cell. An enzyme, catalase, which enzyme activity lab report found in most tissue from living organisms, breaks the substrate H 2 O 2 down into enzyme activity lab report harmless substances, water and oxygen, enzyme activity lab report, according to this equation:.

In this experiment liver will be used as a source of catalase. The oxygen is given off as a gas and, if detergent is added to the substrate, foam is formed, enzyme activity lab report.

The volume of the foam produced in a given time can be used as a measure of the enzyme activity. Our aim in this experiment is to use this reaction to investigate how substrate concentration may influence the rate of the above reaction. We cut 5 cubes of liver each approximately 1cm 3. Using the forceps we took one cube of liver and placed it in the measuring cylinder.

After 30 seconds, enzyme activity lab report, we recorded the volume the foam reached in the cylinder and recorded our data in a table seen below. After that we repeated this procedure with the other 3 solutions of hydrogen peroxide and did the same thing with distilled water. We also made sure we rinsed our glassware carefully between procedures, enzyme activity lab report. Then we obtained results from five other groups and entered them in the table below.

The independent variable during this experiment was the substrate concentration which we used. The dependent variable was the amount of foam formed.

Group nr. Our aim in this experiment has been to investigate how substrate concentration affects the activity of certain enzymes, in this case, catalase. From the results of our experiment, graph A and graph B, we can see clearly that the activity of the reaction taken place increases as we add a more concentrated substrate, H 2 O 2.

When we add water as a substrate, containing no H 2 O 2no reaction is taken place at all. From this we can draw the conclusion that the more concentrated the substrate is, the more reaction will take place with the help of the enzyme catalase, producing more water and oxygen foam. If we compare our results to the IB-study guide, we can observe that the curve of our graph is quite inaccurate.

On the next page is an example of a more correct graph displaying the enzyme activity of increasingly concentrated substrates: Here we can observe that the difference between our results and this example of a correct graph can be summed up into two main points:.

This makes it impossible for an increase of reactions as no more substrate concentration can be broken down without additional enzymes, enzyme activity lab report. The problems and difficulties which caused this inaccuracy in our results might have been caused by several reasons, but the three most obvious limitations of this lab can be summed up in three points:. Different parts of the enzyme activity lab report can also contain different amounts of catalase which again makes this highly inaccurate.

Two drops is not a static volume and is therefore not a precise way of describing an amount of any substance. The amount of detergent increases the amount of foam that is produced so it is very vital during this experiment and has a large effect enzyme activity lab report the result. The density of the foam can differ, enzyme activity lab report thus the volume, which gives us an incorrect appreciation of the reactions taken place.

There are several things we could do to improve this experiment to gain a more accurate result. The catalase can be measured more accurately if the substance is purified in a chemical form, the amount of detergent could have been given with a more exact volume of measurement and finally the reactions taken place could have been measured in a different way than by measuring the amount of foam produced.

During this lab we have investigated how substrate concentration affects the activity of enzymes, in this case catalase. We did this by creating a reaction of the breaking down of a substrate and then recording how much the volume of the product of the reaction differed between different concentrations of the substrate.

The result we got gave us a clear picture of how increased substrate concentration created a dynamic change of the reactions taking place. The result was however very imprecise for several reasons but the one thing which we could still conclude from our results was that the dynamic change that takes place in amounts of reaction when a different substrate concentration is added.

When we compared this to our enzyme activity lab report source we could further conclude that the enzyme activity or the rate of reactions which take place, increase until a certain point when all active sites of the enzymes are occupied, and the rate of the reactions can no longer increase. Skicka en kommentar. Lab Report: Enzyme Activity. Upplagd av The Big Three kl. Etiketter: BiologyOlle. Inga kommentarer:, enzyme activity lab report.

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Enzyme Catalysis Lab Write-Up - Google Документи


enzyme activity lab report


function of the enzyme (some RNA molecules also have catalytic activity). As with any protein, the shape of an enzyme can be affected by different environmental conditions. An increase in temperature can speed up the rate of an enzymatic reaction but it can also change the shape of the. Enzymes. The Rate of Reaction on Liver, Apples, and Potatoes with Hydrogen Peroxide. AP Biology, Mod 5. Abstract The purpose of this lab was to test enzyme function in various environments. Substrate concentration, temperature, and pH all affect the chemical reaction. Lab 2: Enzyme Action (revised Fall ) Experiment 6B: Biology with Vernier Lab 2 - Biol 2 1–Page of 24 Lab 2. Enzyme Action—Effect of Enzyme Concentration, Temperature and pH on Catalase Activity Prelab Assignment Before coming to lab, read carefully the introduction and the procedures for parts I-III, and then.